Changes in the Expression of Pyruvate Dehydrogenase Kinase Isoenzymes During Early Differentiation of Mouse Embryonic Stem Cells
نویسندگان
چکیده
The embryonic stem cells (ESCs) are pluripotent, self-renewing that able to differentiate into any of the germ layers involved in embryogenesis. However, molecular mechanisms control ESC pluripotency and differentiation remain poorly understood. family Pyruvate dehydrogenase kinase (PDK1-4), inactivates mitochondrial pyruvate complex via phosphorylation, plays a crucial role glucose homeostasis. In current study, gene expression levels PDK isoenzymes were analyzed on undifferentiated mouse (mESCs) compared mESCs induced by removal leukemia inhibitory factor (LIF) for 1, 3 5 days. Besides, we performed analysis several genes related differentiation. addition, also determined uptake rates colorimetric assay kit early differentiated mESCs. Differently level pluripotent LIF-depleted suggest they may have roles ESCs. Furthermore, this study lays foundation detailed investigation underlying PDKs transition state
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ژورنال
عنوان ژورنال: Journal of research in veterinary medicine
سال: 2022
ISSN: ['2667-6745']
DOI: https://doi.org/10.30782/jrvm.1131148